Vaccinia Capping Enzyme CAS 56941-23-2

Identification

CAS Number

56941-23-2

EC Number

2.7.7.50

Name

Vaccinia Capping Enzyme

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P) GTP into 80nt transcript within 1h at 37℃ .

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Vaccinia Capping Enzyme CAS 56941-23-2

Product Introduction

This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.

Product Features

Capping rate as high as 95%; the 5′-Cap structure effectively promoting the in vivo expression of mRNA.

Product Features
Product Features

Product Composition

Item No.CompositionStorage Temperature
(℃ )
400U4000U
IVaccinia Capping Enzyme (10 U/μL))-2040μL400μL
Capping Buffer-20 100μL 1000μL
S-adENosylmethionine (SAM 32mM)-20 100μL 1000μL
GTP-20 50μL 500μL

10× Capping Buffer contains: 0.5M Tris-HCl(pH8.0),50mM KCl,10mM MgCl2 and 10mM DTT.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.Quality Control

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).