T7 RNA Polymerase
Optimal reaction temperature
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of [3H] GMP into acid-insoluble precipitate within 1h under the conditions of 37℃ and pH8.
NONH for all modes of transport
Specifications and Other Information of Our T7 RNA Polymerase CAS 9014-24-8
This product is the phage T7 RNA Polymerase derived from recombinant expression in E. coli. It can specifically recognize the T7 promoter sequence, and synthesize RNA complementary to downstream single-stranded DNA of the promoter using the single or double stranded DNA containing the sequence of T7 promoter as the template and NTP as the substrate.
Excellent RNA output rate; homogeneous transcript length; transcript length up to 10K.
|Item No.||Composition||Storage Temperature|
|I||T7RNA Polymerase (50 U/μL)||-20||100μL||1000μL|
1× Buffer contains: 40 mM Tris HCl(pH7.9), 6mM MgCl2, 1mM DTT and 2mM spermidine, 10mM NaCl.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).