T4 RNA ligase CAS UENA-0195

Identification

CAS Number

UENA-0195

EC Number

6.5.1.3

Name

T4 RNA ligase

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to convert 1nM 5’-[32P]rA16 into anti-phosphoric acid form in 30min at 37℃ .

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our T4 RNA ligase CAS UENA-0195

Product Introduction

This product is the ATP-dependent T4RNA Ligase I recombinantly expressed in E. coli. It can catalyze oligonucleotide, single-stranded RNA and DNA intermolecular/ intramolecular 5’-PO4 and 3’-OH to form phosphodiester bond.

Product Composition

Item No.CompositionStorage Temperature
(℃ )
1KU10KU
IT4 RNA Ligase (10U/μL))-200.1mL1mL
10× Reaction Buffer-20 1.5mL15mL
AdENosine-5′-Triphosphate
(ATP 10mM)
-20 0.2mL2mL
PEG 8000-20 1mL10mL

1x Reaction buffer contains 50mM Tris-HCL (pH7.5), 10mM MgCl2 and 1mM DTT.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Usage

Product Usage

• RNA 3′ terminus labelling (using cytidine-3′,5′-[-32P] diphosphate)
• Connecting RNAs
• Synthesizing oligoribonucleotide and oligodeoxyribonucleotide
• Specific modification of tRNA
• Connecting oligodeoxyribonucleotide to single-stranded cDNA to realize 5’RACE (rapid amplification of cDNA termini)
• Site-specific generation of multiplex PCR primer

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).