Poly(A) Polymerase CAS 9026-30-6

Identification

CAS Number

9026-30-6

EC Number

2.7.7.19

Name

Poly(A) Polymerase

Synonyms

Polynucleotide Adenylyltransferase
ATP:polynucleotide adenylyltransferase
ATP RNA Adenylyltransferase
ATP-RNA Adenylyltransferase
Adenylyltransferase, ATP-RNA
Adenylyltransferase, polynucleotide
Poly A Polymerase
Polyadenylate polymerase
Polyadenylate synthetase

MDL Number

MFCD00166322

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of AMP into RNA within 10min at 37℃ in a 20μL reaction system.

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Poly(A) Polymerase CAS 9026-30-6

Product Introduction

This product is the Poly(A) Polymerase recombinantly expressed in E. coli. It catalyzes with ATP as the substrate, and adds adenyl acid to the 3’-hydroxyl terminus of RNA to form the PolyA tail structure in the form of AMP.

Product Features

Efficient mRNA capping: adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for capping). Homogenous tailing length: controlling polyA tail length by adjusting reaction time or enzyme dosage.

Product Features
Product Features

Product Composition

Item No.CompositionStorage Temperature
(℃ )
100U1000U
IPoly(A) Polymerase (50U/μL)-2040μL400μL
Reaction Buffer-20 1.5mL15mL
AdENosine-5’-Triphosphate (ATP10mM)-20 200μL 2000μL

1x Reaction Buffer contains 0.5M Tris-HCl(pH8.0), 50mM KCl and 10mM MgCl2.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).