ATP RNA Adenylyltransferase
Poly A Polymerase
Optimal reaction temperature
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of AMP into RNA within 10min at 37℃ in a 20μL reaction system.
NONH for all modes of transport
Specifications and Other Information of Our Poly(A) Polymerase CAS 9026-30-6
This product is the Poly(A) Polymerase recombinantly expressed in E. coli. It catalyzes with ATP as the substrate, and adds adenyl acid to the 3’-hydroxyl terminus of RNA to form the PolyA tail structure in the form of AMP.
Efficient mRNA capping: adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for capping). Homogenous tailing length: controlling polyA tail length by adjusting reaction time or enzyme dosage.
|Item No.||Composition||Storage Temperature|
|I||Poly(A) Polymerase (50U/μL)||-20||40μL||400μL|
1x Reaction Buffer contains 0.5M Tris-HCl(pH8.0), 50mM KCl and 10mM MgCl2.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).