mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3

Identification

CAS Number

61970-02-3

EC Number

2.1.1.57

Name

mRNA Cap-2′-O-Methyltransferase

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P)GTP into 80nt transcript within 1h at 37℃ .

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3

Product Introduction

This product is the mRNA Cap-2′-O-Methyltransferase of vaccinia virus recombinantly expressed in E. coli. With the RNA of 7-methylguanosine cap (Cap0) as the substrate, it specifically transfers the methyl of methylation donator SAM to the Cap0 structure in RNA to form the Cap1 structure, which can enhance the translation efficiency of mRNA and avoid the innate immune response in certain cells.

Product Features

In vitro capping can avoid in vivo immune response.

Product Features
Product Features

Product Composition

Item No.CompositionStorage Temperature
(℃ )
2KU20KU
ImRNA Cap-2′-O-Methyltransferase(50U/μL)-2040μL400μL
Capping Buffer-20 100μL 1000μL
S-adENosylmethionine (SAM 32mM)-20 100μL 1000μL

10× Capping Buffer contains: 0.5M Tris-HCl(pH8.0), 50mM KCl, 10mM MgCl2 and 10mM DTT.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).