LLCD CAS 9001-60-9

  • Identification
  • Properties
  • Safety Data
  • Specifications & Other Information
  • Links
  • Identification

    CAS Number

    9001-60-9

    EC

    1.1.1.27

    Name

    LLCD, L-Lactic dehydrogenase

    Source

    Microorganism

    Molecular weight

    38 kDa (SDS-PAGE)

    EINECS

    232-617-8

    Properties

    Appearance

    White amorphous powder

    Isoelectric point

    6.2

    Michaelis constant

    1.3×10 M(Pyruvate)
    -6 4.0×10 M(NADH)

    Safety Data

    RIDADR 

    NONH for all modes of transport

    WGK Germany

    3

    Specifications and Other Information of Our LLCD CAS 9001-60-9

    Specific activity

    ≥ 300U/mg protein

    Unit definition

    One unit will convert one micromole of pyruvate to L-lactate per min at pH 7.4 at 25℃.

    Optimum pH

    6.5

    Optimum temperature

    45℃

    pH Stability

    4.5~10.0 (37℃,1hr)

    Thermal stability

    < 50℃ (pH 7.4, 15min)

    Inhibitors

    Co ,Cu ,Fe ,Ni ,Zn ,NEM,SDS,Proclin

    Effect of Various Chemicals on L-LDH

    The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr

    ChemicalConcn.(mM)Residual activity
    None2.0100%
    CaCl22.0101%
    CoCl22.050%
    CuSO42.00%
    FeCl32.027%
    MgSO42.094%
    MnSO42.096%
    NiCl22.078%
    ZnSO42.00%
    K4Fe(CN)62.098%
    BME2.098%
    NME2.079%
    EDTA5.0102%
    NaN320.0101%
    Proclin0.045%58%
    Na-cholate0.10%109%
    SDS0.05%0%
    Triton X-1000.10%113%
    Tween 200.10%115%

    Shelf Life

    2 years

    Storage

    Store at -20℃ for long time.

    Known Application

    For biochemical research, determination of pyruvate, diagnosis of leukemia and myocardial infarction.

    This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).