Ascorbate oxidase CAS UENA-0244
Identification
CAS Number
UENA-0244
Name
Ascorbate oxidase
Synonyms
ASCORBASE;
ASCORBATE OXIDASE;
ASCORBATE OXIDASE CUCURBITA SPECIES;
EC 1.10.3.3;
L-ASCORBATE: OXYGEN OXIDOREDUCTASE;
Oxidase, ascorbate;
ASCORBATE OXIDASE FROM CUCURBITASPECIES
Molecular weight
80 kDa (Gel filtration)
Properties
Appearance
Light blue amorphous powder, lyophilized
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Ascorbate oxidase CAS UENA-0244
Activity
≥100 U/mg solid
Km
Ascorbic acid (pH 7.0) 1.0×10-4 M
Ascorbic acid (pH 4.0) 3.8×10-4 M
Optimum pH
4.0–4.5 (Acetate buffer)
Optimum temperature
55℃
pH stability
6.0–9.0 (30℃, 24 hr)
Thermal stability
Stable at 50 ℃ and below (pH 7.0,10 min)
SOL. (50mg/ml water)
Corresponds
PH (50mg/ml water)
7.5
ACT. (25°C; with L-ascorbate)
178 u/mg L
SPEC. ACT. (25°C; with L-ascorbate)
2119 u/mg P
ACT. (37°C; with L-ascorbate)
278 u/rng L
SPEC. ACT. (37°C; with L-ascorbate)
3471 u/mg P
PROTEIN (BCA reag.)
0.084 mg/mg L
Contaminating Activities
Corresponds
GOT
<0.00005%
GPT
<0.00006%
Catalase
<0.008%
Contaminating Oxidases
<0.00003%
Storage stability
-20 ℃ for 12 months for more than 90% activity.
Storage
Store at -20°C for long time, sealed and away from light.
Known Application
This enzyme is mainly used to effectively prevent the interference of ascorbic acid on diagnostic tests such as blood, uric acid, TG, TC, and creatinine.
Links
This product is developed by our R&D company Watsonnoke Scientific Ltd (http://www.watsonnoke.com/).