RNase III CAS UENA-0194

RNase III CAS UENA-0194

Identification

CAS Number

UENA-0194

EC Number

3.1.26.3

Name

RNase III

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μg of dsRNA into siRNA in 20min at 37℃ in a 50μL reaction system.

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our RNase III CAS UENA-0194

Product Introduction

This product is the RNase Ⅲ recombinantly expressed in E. coli. This specific exonuclease is capable of cutting double-stranded RNA (dsRNA) and generating 12-35bp dsRNA fragments with protruding 5′-PO4, 3′-OH, and 3’ termini.

Product Features

Efficient production of siRNA; accurate interference with target gene expression.

Product Features
Product Features

Product Composition

Item No.CompositionStorage Temperature
(℃ )
200U2000U
IRNase III (2U/μL)-200.5mL5mL
10x Reaction Buffer-20 1.5mL15mL
10x EDTA-20 1mL10mL
10x MnCl2-20 1mL10mL

10× Reaction buffer contains 500mM Tris-HCl(pH7.5)500mM NaCl and 10mM DTT.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).