Optimal reaction temperature
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μg of dsRNA into siRNA in 20min at 37℃ in a 50μL reaction system.
NONH for all modes of transport
Specifications and Other Information of Our RNase III CAS UENA-0194
This product is the RNase Ⅲ recombinantly expressed in E. coli. This specific exonuclease is capable of cutting double-stranded RNA (dsRNA) and generating 12-35bp dsRNA fragments with protruding 5′-PO4, 3′-OH, and 3’ termini.
Efficient production of siRNA; accurate interference with target gene expression.
|Item No.||Composition||Storage Temperature|
|I||RNase III (2U/μL)||-20||0.5mL||5mL|
|Ⅱ||10x Reaction Buffer||-20||1.5mL||15mL|
10× Reaction buffer contains 500mM Tris-HCl(pH7.5)500mM NaCl and 10mM DTT.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).