5’-Nucleotidase CAS 9027-73-0

Identification

CAS Number

9027-73-0

Name

5’-Nucleotidase

Synonyms

5′-NUCLEOTIDASE FROM CROTALUS ATROX VENOM;
5′-nucleotidase from crotalus adamanteus venom;
Nucleotidase, 5′-;5′-NT;5′-Nucleotidase human ;
CD73;
ecto-5′-nucleotidase;
NT5E

Molecular Formula

232-820-1

Molecular Weight

447.225 

MDL Number

MFCD00130311

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our
5’-Nucleotidase CAS 9027-73-0

General Assay Characteristics

Method
Enzymatic/RATE A/E
ReagentsReady to use
CalibratorsLevel 1-0.0 U/L; Level 2-60 U/L
Calibration Stability28 days
Sample TypeEDTA,SST,Serum,Heparinised plasma
Assay Range1-100U/L undiluted
Standardisation

Assay Principle

Proposed 5’-NT Recipe

Reagent I

CompositionConcentrationCatalog Number
Tris-HCl buffer50mM
Xanthine Oxidase>1KU/LEM022
4-Aminoantipyrine1mM
Purine Nucleoside>1KU/LEM021
Phosphorylase
POD>1KU/L
Detergent, Preservative

Reagent II

CompositionConcentrationCatalog Number
Tris-HCl buffer50mM
5’-IMP10mM
TOOS10mM
EDTA8mM
Detergent, Preservative

Major reagent ingredients made by Assay Specilist Lab are list on right

Standard&Control

Home Made: 5’-NT(CC001) in buffer

Reaction curve chart

Known Application

Commonly used in biochemical research, food preservation, clinically used as a diagnostic enzyme, determination of phosphoric acid and creatine phosphate.

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